Daphne after shoots10/28/2022 ![]() On the other hand, the content of dry matter in shoots did not significantly differ between tested media, and amounted to 14–15% (tab. Shoots obtained on tested media differed significantly in fresh matter content, and the highest amount was noted in shoots from medium with 1.0 mM Pb(NO 3 ) 2. Higher doses of lead nitrate slightly inhibited shoot prolif- eration (obtained MC ranged from 3.2 to 3.3), as well as growth of shoots (approx. 50% of shoot height in control) and had the lowest fresh weight. Also, on the medium with 0.1 Pb(NO 3 ) 2 shoots have been the shortest (approx. On medium with 0.1 mM Pb(NO 3 ) 2 micropropagation coefficient decreased to 2.4, in comparison to MC = 3.9 on control medium. Interestingly, lead nitrate in its lowest concentration tested occurred to be the most inhibitory. tangutica, proliferated less vigorously on the media containing various doses of Pb(NO 3 ) 2 than on the control medium (tab. ![]() The second of examined Daphne species, D. On the remaining media no macroscopic stages of rhizogenesis were observed. During the experiment, on medium containing 0.1 Pb(NO 3 ) 2 small root primordia ( 1 mm) developed on the shoot base. Regenerated roots were short (mean length = 8.2 mm) and had approximately 12% of dry biomass content. Mean number of roots per microcutting accounted to 8.9 ☑.8 (data not shown). Development of root system was observed on propagation medium without addition of lead nitrate. Irrespective of applied medium, shoots were properly colored, with no signs of leaf chlorosis. Although biomass production decreased on all tested lead nitrate concentrations in comparison to control treatments, in the course of experiment proliferative shoot cultures were established on all media containing Pb(NO 3 ) 2. Interestingly, growth tolerance index (GTI) was the highest on medium containing 1.0 mM lead nitrate and reached 89.5%, lower doses of the salt resulted in significant inhibition of shoot dry biomass production (to approx. The content of shoot dry biomass significantly increased on media with Pb ions. The lowest value, which was about 72% of the control, was noted for shoots obtained on medium with 0.5 mM (tab. The presence of lead nitrate in the medium negatively affected fresh weight of shoots. On Pb-supplemented media shoots were shorter in comparison with the control treatment (no Pb ions), irrespectively of given Pb(NO 3 ) 2 concentration. Respective values of micropropagation coefficient varied from 3.4 (control) to 2.7, however the differences between treatments were found to be statistically insignificant (tab. Multiplication rate of Daphne jasminea shoots obtained on media containing lead nitrate were proved to be rather similar to that from medium without Pb ions (control medium). The post-hoc Duncan’s test was used to study differences between treatments at P < 0.05. the accumulation of pigments, and the content of lead and of other chemical elements were subjected to ANOVA analysis (STATISTICA, StatSoft, Tulsa, OK, USA). In the parking strip on the west side on 10th St. ![]() ![]() genkwa: "a transliteration of the Japanese pronunciation of its Chinese name" (Valder, 1999), which is Yuan Hua (Yuan Flower).Caution: reportedly all parts of the plant are poisonous, and it has been used as a pesticide dried flower buds are used in some Chinese medicine. ![]()
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